Transcriptomic profiling with RNA-seq is increasingly common. Often, RNA-seq is used a first step to discover genes, followed by manipulation of said genes to show a causal relationship with some phenotype. That’s often the case, but sometimes, just the transcriptomic data are reported without any follow-up perturbations. The volume of data one gets from transcriptomic …
Tan, L. L. et al. A pathway from midcingulate cortex to posterior insula gates nociceptive hypersensitivity. Nat Neurosci 4, 362 (2017). This is a beautiful and insightful paper from Rohini Kuner’s lab. I’m using this as an exemplary model of how to causally implicate a brain region in some kind of pain behavior. The general …
There is a very large variety of kinds of research approaches in pain neuroscience, and science in general. If you read enough papers, you can begin to glean ‘models’ or templates for how certain kinds of research gets done. I’m starting this series to begin to describe and catalog examples of different models of scientific …